Transcript

The Oregon State University Honeybee Lab
presents dissecting for tracheal mites.
To determine if a bee is infested with
tracheal mites, one must dissect the bee
to view tracheal tubes.
Place the bee on its back with forceps.
Remove the head and first pair of legs.
Pull on the collar of the thorax and
remove. The collar may be brittle and
break apart, so the technique may take
practice.
Trachea should be attached to the collar
piece or they may still be attached to
the bee body.
This is an adult tracheal mite and an
egg that have been removed with forceps
from the trachea. They are small, white,
and football-shaped.
This is a tracheal tube with no tracheal
mites.
This is a mild infestation of tracheal
mites. This is a moderate infestation.
This is a heavy infestation of
trachomites.
Severe infections may cause dark
scarring of the trachea. However,
scarring can occur with different levels
of infestation.

Tracheal mites (Acarapis woodi) infest and breed in the tracheae of honey bees. (Tracheae are fine tubes in the insect’s body that convey air to the tissues.) It’s not possible to diagnose a tracheal mite infestation without microscopic dissection.

In this video, we demonstrate the dissection of a honey bee worker for tracheal mite infestation. This technique is based on that of Dr. Diana Sammataro of USDA-ARS. The best time to sample for tracheal mites is early spring or when symptoms appear and you suspect an infestation. A dissecting microscope with good lighting is necessary, as are very fine forceps for dissection.

Colony level symptoms are not reliable for diagnosing tracheal mite infestation. That said, symptoms may include failure to thrive, bees crawling in near the entrance of the colony, and wings in a “K” formation. Symptoms also may be confused with other colony level problems such as parasitic mite syndrome or pesticide exposure.

Knowing the levels of tracheal mites in your apiary and understanding the effects of their presence will enable you to make an educated decision on your treatment plan. Keeping pests and pathogens in check is necessary for strong, healthy honey bee colonies. For help with a diagnosis, please contact the Oregon State University Honey Bee Lab for more information: (541) 737-1447.

References: Sammataro, D. 2006. An easy dissection technique for finding the tracheal mite, Acarapis woodi (Rennie) (Acari: Tarsonemidae), in honey bees, with video link. Internat. J. Acarol. 32 (4): 1-5.

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